Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock
Present address: Malaria Research Institute, John Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85°C to 65°C and 95°C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95°C included upregulation of genes encoding chaperones, and downregulation of genes encoding subunits of the H+ transporting ATP synthase. A gene encoding an α subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in M. jannaschii. Very different responses were observed upon cold shock to 65°C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP-type PPIase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions.
2002) Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus.
1996) Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.
1999) Trigger factor and DnaK cooperate in folding of newly synthesized proteins.
2002) A membrane-bound archaeal lon protease displays ATP-independent proteolytic activity towards unfolded proteins and ATP-dependent activity for folded proteins.
2000) Genomic expression programs in the response of yeast cells to environmental changes.
Mol Biol Cell
2002) Phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling.
FEMS Microbiol Lett
2004) A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii.
2002) Molecular chaperones in the cytosol: from nascent chain to folded protein.
1992) Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule.
J Mol Biol
2002) Expression of long- and short-type FK506 binding proteins in hyperthermophilic archaea.
2002) Identification of genes that are associated with DNA repeats in prokaryotes.
1983) Methanococcus jannaschii sp. nov., an extremely thermophilic methanogen from a submarine hydrothermal vent.
2001) Protein folding and molecular chaperones in archaea.
Adv Appl Microbiol
1999) MtGimC, a novel archaeal chaperone related to the eukaryotic chaperonin cofactor GimC/prefoldin.
2000) Low temperature regulated DEAD-box RNA helicase from the Antarctic archaeon, Methanococcoides burtonii.
J Mol Biol
1995) Crystal-structure of the 20s proteasome from the archaeon T-acidophilum at 3.4-angstrom resolution.
1988) Pressure and temperature effects on growth and methane production of the extreme thermophile Methanococcus jannaschii.
Appl Environ Microbiol
1999) AAA(+): a class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes.
2002) Pyrococcus prefoldin stabilizes protein-folding intermediates and transfers them to chaperonins for correct folding.
Biochem Biophys Res Commun
2001) The comprehensive microbial resource.
Nucleic Acids Res
2004) Transcriptional analysis of dynamic heat-shock response by the hyperthermophilic bacterium Thermotoga maritima.
1997) Methanogenesis: genes, genomes, and who's on first?
2001) The chaperones of the archaeon Thermoplasma acidophilum.
J Struct Biol
2003) TM4: a free, open-source system for microarray data management and analysis.
2003) Heat shock response by the hyperthermophilic archaeon Pyrococcus furiosus.
Appl Environ Microbiol
2003) Gene expression profile of Campylobacter jejuni in response to growth temperature variation.
2001) Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii.
Proc Natl Acad Sci USA
2001) DExD/H box RNA helicases: from generic motors to specific dissociation functions.
1994) Continuous-culture of Methanococcus-jannaschii, an extremely thermophilic methanogen.
1995) NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center.
J Cell Biol
2001) Gonadotropin-releasing hormone receptor-coupled gene network organization.
J Biol Chem