Volume 7, Issue 6 p. 789-797

Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock

Boonchai B. Boonyaratanakornkit,

Boonchai B. Boonyaratanakornkit

Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.

Boonchai B. Boonyaratanakornkit and Anjana J. Simpson contributed equally to this work.

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Anjana J. Simpson,

Anjana J. Simpson

The Institute for Genomic Research, Rockville, MD 20850, USA.

Boonchai B. Boonyaratanakornkit and Anjana J. Simpson contributed equally to this work.

Present address: Malaria Research Institute, John Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.

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Timothy A. Whitehead,

Timothy A. Whitehead

Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.

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Claire M. Fraser,

Claire M. Fraser

The Institute for Genomic Research, Rockville, MD 20850, USA.

Department of Microbiology and Tropical Medicine, George Washington University, Washington, DC 20052, USA.

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Najib M. A. El-Sayed,

Najib M. A. El-Sayed

The Institute for Genomic Research, Rockville, MD 20850, USA.

Department of Microbiology and Tropical Medicine, George Washington University, Washington, DC 20052, USA.

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Douglas S. Clark,

Corresponding Author

Douglas S. Clark

Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.

E-mail [email protected]; Tel. (+1) 510 642 2408; Fax (+1) 510 643 1228.Search for more papers by this author

Boonchai B. Boonyaratanakornkit,

Boonchai B. Boonyaratanakornkit

Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.

Boonchai B. Boonyaratanakornkit and Anjana J. Simpson contributed equally to this work.

Search for more papers by this author

Anjana J. Simpson,

Anjana J. Simpson

The Institute for Genomic Research, Rockville, MD 20850, USA.

Boonchai B. Boonyaratanakornkit and Anjana J. Simpson contributed equally to this work.

Present address: Malaria Research Institute, John Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.

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Timothy A. Whitehead,

Timothy A. Whitehead

Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.

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Claire M. Fraser,

Claire M. Fraser

The Institute for Genomic Research, Rockville, MD 20850, USA.

Department of Microbiology and Tropical Medicine, George Washington University, Washington, DC 20052, USA.

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Najib M. A. El-Sayed,

Najib M. A. El-Sayed

The Institute for Genomic Research, Rockville, MD 20850, USA.

Department of Microbiology and Tropical Medicine, George Washington University, Washington, DC 20052, USA.

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Douglas S. Clark,

Corresponding Author

Douglas S. Clark

Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.

E-mail [email protected]; Tel. (+1) 510 642 2408; Fax (+1) 510 643 1228.Search for more papers by this author

First published: 07 March 2005

https://doi.org/10.1111/j.1462-2920.2005.00751.x

Citations: 49

Present address: Malaria Research Institute, John Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.

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Summary

Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85°C to 65°C and 95°C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95°C included upregulation of genes encoding chaperones, and downregulation of genes encoding subunits of the H⁺ transporting ATP synthase. A gene encoding an α subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in M. jannaschii. Very different responses were observed upon cold shock to 65°C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP-type PPIase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions.

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Volume7, Issue6

June 2005

Pages 789-797

Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock

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Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock

Summary

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