Volume 74, Issue 4 p. 593-603
Original Article

Biological characteristics and whole-genome analysis of the potential probiotic, Lactobacillus reuteri S5

Shuiqin Shi

Shuiqin Shi

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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J. Dong

J. Dong

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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X. Cheng

X. Cheng

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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J. Hu

J. Hu

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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Y. Liu

Y. Liu

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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G. He

G. He

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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J. Zhang

J. Zhang

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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H. Yu

H. Yu

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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L. Jia

L. Jia

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

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D. Zhou

Corresponding Author

D. Zhou

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing, P.R. China

Correspondence

Duoqi Zhou, School of Life Sciences, 1318 North Jixian Road, Anqing, Anhui 246133, P.R. China.

E-mail: [email protected]

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First published: 11 January 2022

Abstract

Lactic acid bacteria are micro-organisms used for probiotic purposes and form major parts of human and mammalian intestinal microbiota, exerting important health-promoting effects on the host. Here, we evaluated Lactobacillus reuteri strain S5 isolated from the intestines of healthy white feather broilers. Lactobacillus reuteri S5 grew best after 20 h of incubation in MRS medium. Lactic acid production was 1·42 mmol l−1 at 24 h, which was well tolerated. Activities of T-AOC, GSH-Px and T-SOD in the cell-free fermentation supernatant of L. reuteri S5 were higher than those in the bacteria, and the strain showed good hydrophobicity in vitro. The dominant carbon and nitrogen sources of L. reuteri S5 were glucose and soybean meal. A high-quality complete genome map of L. reuteri S5 was obtained using a Pacbio nanopore third-generation sequencing platform. The results showed that L. reuteri S5 possesses a complete primary metabolic pathway, encoding the main functional enzymes of the glycolysis pathway and pentose phosphate pathway. The genome contains genes encoding antioxidants and conferring tolerance to inorganic salt ions, acids and bile salts. This study shows that L. reuteri S5 is a probiotic strain with excellent probiotic characteristics and has great potential for the development of feed additives to promote animal health.

Conflict of Interest

The author has no conflict of interest to declare.

Data availability statement

The data used to support the findings of this study are available from the corresponding author upon request.